Generator

Part:BBa_J70636:Design

Designed by: Tom Knight   Group: Knight Lab   (2010-11-01)


pheS gene from M. florum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1005
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none.


Source

PCR from M. florum genomic DNA. Primers:

  • ttccgaattcgcggccgcttctagag ctctgttattggtatagtta,pheS-mf-F2
  • ttccactagtatgttctacctactctcc,pheS-mf-R



References