Generator
Part:BBa_J70636:Design
Designed by: Tom Knight Group: Knight Lab (2010-11-01)
pheS gene from M. florum
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1005
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
none.
Source
PCR from M. florum genomic DNA. Primers:
- ttccgaattcgcggccgcttctagag ctctgttattggtatagtta,pheS-mf-F2
- ttccactagtatgttctacctactctcc,pheS-mf-R